Classical analgesic drugs modulate nociceptive-like escape behavior in Drosophila melanogaster larvae
نویسندگان
چکیده
Introduction: Nociceptive stimulus triggers escape responses in Drosophila melanogaster larvae, characterized by 360° rolling behavior along its own body axis. Therefore, it is possible to study analgesic drugs based on this stereotypical nociceptive-like behavior. Here, we aimed develop an predictive validity test of thermal nociception through D. larvae.
 Materials and methods: We evaluated the effect classical analgesics (morphine, dipyrone, acetylsalicylic acid (ASA) dexamethasone (DXM)) latency larvae exposed thermal-acute noxious nociceptive sensitization paradigm. Drugs were injected into hemocoel (100 nL) before nociceptive measurement.
 Results discussion: Rolling was increased morphine (2, 4, 8 16 ng) dose-dependent manner. Naloxone (4 fully reversed maximum morphine. Dipyrone (32, 64 128 DXM (8 elicited antinociceptive effects. Exposure 97% maximal infrared intensity induced sensitization, i.e., changed from 12 7.5 seconds. ASA (25, 50 100 (4, administered 150 min after displayed reverse rapid onset (30 injection). (16 ng), prior a delay action (150 Locomotor behaviors not affected substances.
 Conclusion: Our findings open perspectives for evaluation discovery using melanogaster model.
 Graphical abstract:
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ژورنال
عنوان ژورنال: Research results in pharmacology
سال: 2022
ISSN: ['2658-381X']
DOI: https://doi.org/10.3897/rrpharmacology.8.91390